---
name: SolubleMPNN
slug: solublempnn
category: AI Engineering
description: SolubleMPNN designs protein sequences with a soluble model variant for E. coli expression and higher solubility. Use it to reduce aggregation or inclusion bodies when standard ProteinMPNN is too hydrophobic.
github: "https://github.com/BioTender-max/awesome-bio-agent-skills/tree/main/skills/adaptyv/solublempnn"
language: Python
stars: 174
forks: 30
install: "npx degit https://github.com/BioTender-max/awesome-bio-agent-skills/tree/main/skills/adaptyv/solublempnn ~/.claude/skills/solublempnn"
installs_to: ~/.claude/skills/solublempnn
source_path: skills/adaptyv/solublempnn/SKILL.md
collection_size: 25
category_size: 3475
collection_url: "https://dirskills.com/collections/BioTender-max/awesome-bio-agent-skills"
added: 2026-09-07T05:21:59.118Z
last_synced: 2026-09-07T05:21:59.118Z
canonical_url: "https://dirskills.com/skills/solublempnn"
---

# SolubleMPNN

SolubleMPNN designs protein sequences with a soluble model variant for E. coli expression and higher solubility. Use it to reduce aggregation or inclusion bodies when standard ProteinMPNN is too hydrophobic.

**Install:**

```bash
npx degit https://github.com/BioTender-max/awesome-bio-agent-skills/tree/main/skills/adaptyv/solublempnn ~/.claude/skills/solublempnn
```

## README

# SolubleMPNN Solubility-Optimized Design

## Prerequisites

| Requirement | Minimum | Recommended |
|-------------|---------|-------------|
| Python | 3.8+ | 3.10 |
| CUDA | 11.0+ | 11.7+ |
| GPU VRAM | 8GB | 16GB (T4) |
| RAM | 8GB | 16GB |

## How to run

> **First time?** See [Installation Guide](../../docs/installation.md) to set up Modal and biomodals.

### Option 1: Modal (recommended)
SolubleMPNN uses the ProteinMPNN Modal wrapper with soluble model:
```bash
cd biomodals
modal run modal_proteinmpnn.py \
  --pdb-path backbone.pdb \
  --num-seq-per-target 16 \
  --sampling-temp 0.1 \
  --model-name v_48_020
```

**GPU**: T4 (16GB) | **Timeout**: 600s default

### Option 2: Local installation
```bash
git clone https://github.com/dauparas/ProteinMPNN.git
cd ProteinMPNN

# Use soluble model weights
python protein_mpnn_run.py \
  --pdb_path backbone.pdb \
  --out_folder output/ \
  --num_seq_per_target 16 \
  --sampling_temp "0.1" \
  --model_name "v_48_020"  # Soluble model
```

## Key parameters

| Parameter | Default | Range | Description |
|-----------|---------|-------|-------------|
| `--pdb_path` | required | path | Input structure |
| `--num_seq_per_target` | 1 | 1-1000 | Sequences per structure |
| `--sampling_temp` | "0.1" | "0.0001-1.0" | Temperature (string!) |
| `--model_name` | v_48_020 | string | Soluble model variant |

## Model Variants

| Model | Description | Use Case |
|-------|-------------|----------|
| v_48_002 | Standard | General design |
| v_48_020 | Soluble-trained | E. coli expression |
| v_48_030 | High solubility | Difficult targets |

## Output format

```
output/
├── seqs/backbone.fa
└── backbone_pdb/backbone_0001.pdb
```

## Sample output

### Successful run
```
$ python protein_mpnn_run.py --pdb_path backbone.pdb --model_name v_48_020 --num_seq_per_target 8
Loading soluble model weights (v_48_020)...
Designing sequences for backbone.pdb
Generated 8 sequences in 2.1 seconds

output/seqs/backbone.fa:
>backbone_0001, score=1.31, global_score=1.24, seq_recovery=0.78
MKTAYIAKQRQISFVKSHFSRQLE...
>backbone_0002, score=1.28, global_score=1.21, seq_recovery=0.81
MKTAYIAKQRQISFVKSQFSRQLD...
```

**What good output looks like:**
- Score: 1.0-2.0 (lower = more confident)
- Reduced hydrophobic patches compared to standard MPNN
- Improved charge distribution

## Decision tree

```
Should I use SolubleMPNN?
│
├─ What expression system?
│  ├─ E. coli → SolubleMPNN ✓
│  ├─ Mammalian → ProteinMPNN (PTMs matter more)
│  └─ Yeast → Either
│
├─ History of expression problems?
│  ├─ Yes, aggregation → SolubleMPNN ✓
│  ├─ Yes, low yield → SolubleMPNN ✓
│  └─ No → ProteinMPNN is fine
│
├─ What's in the binding site?
│  ├─ Small molecule / ligand → Use LigandMPNN
│  └─ Nothing / protein only → SolubleMPNN ✓
│
└─ Need highest solubility?
   ├─ Yes → Use v_48_030 model
   └─ Standard → Use v_48_020 model
```

## Typical performance

| Campaign Size | Time (T4) | Cost (Modal) | Notes |
|---------------|-----------|--------------|-------|
| 100 backbones × 8 seq | 15-20 min | ~$2 | Standard |
| 500 backbones × 8 seq | 1-1.5h | ~$8 | Large campaign |

**Expected improvement**: +15-30% solubility score vs standard ProteinMPNN.

---

## Verify

```bash
grep -c "^>" output/seqs/*.fa  # Should match backbone_count × num_seq_per_target
```

---

## Troubleshooting

**Still insoluble**: Try v_48_030 (higher solubility bias)
**Low diversity**: Increase temperature to 0.2
**Poor folding**: Use standard ProteinMPNN and optimize later

### Error interpretation

| Error | Cause | Fix |
|-------|-------|-----|
| `RuntimeError: CUDA out of memory` | Long protein or large batch | Reduce batch_size |
| `FileNotFoundError: v_48_020` | Missing model weights | Download soluble weights |

---

**Next**: Structure prediction for validation → `protein-qc` for filtering.